sa β gal Search Results


senescence β-galactosidase staining kit g1580  (Beijing Solarbio Science)
90
Beijing Solarbio Science senescence β-galactosidase staining kit g1580
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Senescence β Galactosidase Staining Kit G1580, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pmc10811476-321-15-20?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
senescence β-galactosidase staining kit g1580 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sa-β-gal a1007,0001  (Applichem inc)
90
Applichem inc sa-β-gal a1007,0001
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Sa β Gal A1007,0001, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pm38649488-722-24-25?v=Applichem+inc
Average 90 stars, based on 1 article reviews
sa-β-gal a1007,0001 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
beta-gal assay kit  (Beijing Solarbio Science)
90
Beijing Solarbio Science beta-gal assay kit
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Beta Gal Assay Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pm37867213-474-15-18?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
beta-gal assay kit - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sa-β-gal staining kit b8050  (Beijing Solarbio Science)
90
Beijing Solarbio Science sa-β-gal staining kit b8050
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Sa β Gal Staining Kit B8050, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pm40169516-38-5-11?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
sa-β-gal staining kit b8050 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sa-b-gal staining kit solarbio, beijing, china  (Beijing Solarbio Science)
90
Beijing Solarbio Science sa-b-gal staining kit solarbio, beijing, china
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Sa B Gal Staining Kit Solarbio, Beijing, China, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pm39490613-83-1-4?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
sa-b-gal staining kit solarbio, beijing, china - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sa-β-gal activity  (Gallus BioPharmaceuticals)
90
Gallus BioPharmaceuticals sa-β-gal activity
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Sa β Gal Activity, supplied by Gallus BioPharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pm38935557-104-14-11?v=Gallus+BioPharmaceuticals
Average 90 stars, based on 1 article reviews
sa-β-gal activity - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sa-β-gal staining solution  (Beijing Solarbio Science)
90
Beijing Solarbio Science sa-β-gal staining solution
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Sa β Gal Staining Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pmc08175795-67-18-21?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
sa-β-gal staining solution - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
protocol for measuring the chromatic (sa-β-gal) dye staining  (CompuCyte Corporation)
90
CompuCyte Corporation protocol for measuring the chromatic (sa-β-gal) dye staining
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Protocol For Measuring The Chromatic (Sa β Gal) Dye Staining, supplied by CompuCyte Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pmc03796215-171-5-12?v=CompuCyte+Corporation
Average 90 stars, based on 1 article reviews
protocol for measuring the chromatic (sa-β-gal) dye staining - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sa-β-gal  (Beijing Solarbio Science)
90
Beijing Solarbio Science sa-β-gal
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Sa β Gal, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pm34583621-66-16-22?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
sa-β-gal - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sa β-gal staining  (Merck KGaA)
90
Merck KGaA sa β-gal staining
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Sa β Gal Staining, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pmc09864220-123-1-12?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
sa β-gal staining - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sa- β -gal fixation solutions  (Beijing Solarbio Science)
90
Beijing Solarbio Science sa- β -gal fixation solutions
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Sa β Gal Fixation Solutions, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/pmc08728814-222-0-5?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
sa- β -gal fixation solutions - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sa-β-gal staining  (MetaMorph Inc)
90
MetaMorph Inc sa-β-gal staining
Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of <t>senescence‐associated</t> <t>β‐galactosidase</t> (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Sa β Gal Staining, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa+%CE%B2+gal/10__1172_slash_jci70559-103-12-17?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
sa-β-gal staining - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of senescence‐associated β‐galactosidase (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.

Journal: Advanced Science

Article Title: Single‐Cell Atlas of Human Ovaries Reveals The Role Of The Pyroptotic Macrophage in Ovarian Aging

doi: 10.1002/advs.202305175

Figure Lengend Snippet: Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of senescence‐associated β‐galactosidase (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.

Article Snippet: SA‐beta‐gal staining was performed on primary human ovarian stromal cells (iCell Bioscience Inc, HUM‐iCell‐f001) using Senescence β‐Galactosidase Staining Kit (G1580, Solarbio, G1580).

Techniques: Immunostaining, Expressing, Activity Assay, Staining

Pyroptotic macrophages promote stromal cell senescence. A) Representative images of senescence‐associated β‐galactosidase (SA‐beta‐gal) staining of stromal cells treated with the supernatant of TNF‐α/IFN‐γ‐stimulated THP1 cells, the supernatant of LPS/Nig‐stimulated THP1 cells or the supernatant of unstimulated THP1 cells (scale bar, 500 µm). Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image. B) The relative expression of fibrosis‐related genes in ovarian stromal cells treated with supernatant of unstimulated or stimulated THP1 cells. The results were analyzed by ANOVA. C) Representative IHC staining for GSDMD showing the elevated expression of GSDMD in the middle‐aged group (scale bar, 100 µm). The insert at top left corner of each image is the magnified image (scale bar, 250 µm). D) EREG concentration in follicular fluids measured by ELISA. E) Flow cytometric analysis of follicular fluids from IVF patients: representative flow cytometric dot plots of macrophages in follicular fluids from each group. Statistical graph of the percentages of macrophages in follicular fluids. F) Representative histogram plots and the statistical graph of the MFI of GSDMD. MFI: mean fluorescence intensity.

Journal: Advanced Science

Article Title: Single‐Cell Atlas of Human Ovaries Reveals The Role Of The Pyroptotic Macrophage in Ovarian Aging

doi: 10.1002/advs.202305175

Figure Lengend Snippet: Pyroptotic macrophages promote stromal cell senescence. A) Representative images of senescence‐associated β‐galactosidase (SA‐beta‐gal) staining of stromal cells treated with the supernatant of TNF‐α/IFN‐γ‐stimulated THP1 cells, the supernatant of LPS/Nig‐stimulated THP1 cells or the supernatant of unstimulated THP1 cells (scale bar, 500 µm). Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image. B) The relative expression of fibrosis‐related genes in ovarian stromal cells treated with supernatant of unstimulated or stimulated THP1 cells. The results were analyzed by ANOVA. C) Representative IHC staining for GSDMD showing the elevated expression of GSDMD in the middle‐aged group (scale bar, 100 µm). The insert at top left corner of each image is the magnified image (scale bar, 250 µm). D) EREG concentration in follicular fluids measured by ELISA. E) Flow cytometric analysis of follicular fluids from IVF patients: representative flow cytometric dot plots of macrophages in follicular fluids from each group. Statistical graph of the percentages of macrophages in follicular fluids. F) Representative histogram plots and the statistical graph of the MFI of GSDMD. MFI: mean fluorescence intensity.

Article Snippet: SA‐beta‐gal staining was performed on primary human ovarian stromal cells (iCell Bioscience Inc, HUM‐iCell‐f001) using Senescence β‐Galactosidase Staining Kit (G1580, Solarbio, G1580).

Techniques: Staining, Expressing, Immunohistochemistry, Concentration Assay, Enzyme-linked Immunosorbent Assay, Fluorescence